Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection

Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection

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Successful generation of virions from infected cells is a complex process requiring turbo air m3f24-1-n orchestrated regulation of host and viral genes.Cells infected with human cytomegalovirus (HCMV) undergo a dramatic reorganization of membrane organelles resulting in the formation of the virion assembly compartment, a process that is not fully understood.Here we show that acidification of vacuoles by the cellular v-ATPase is a crucial step in the formation of the virion assembly compartment and disruption of acidification results in mis-localization of virion components and a profound reduction in infectious virus levels.In addition, knockdown of ATP6V0C blocks the increase in nuclear size, normally associated with HCMV infection.Inhibition of the v-ATPase does not affect intracellular levels of viral DNA synthesis or gene expression, consistent with a defect in assembly and egress.

These studies identify a novel hellfire sloe gin host factor involved in virion production and a potential target for antiviral therapy.